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PERBANDINGAN HASIL PEMERIKSAAN ANTIBODI Human Immunodeficiency Virus (HIV) DENGAN METODE Enzyme-Linked Immunosorbent Assay (ELISA) dan IMMUNOCHROMATOGRAPHY (ICT)
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ABSTRAK
Perbandingan Hasil Pemeriksaan Antibodi Human Immunodeficiency Virus
(HIV) Dengan Metode Enzyme-Linked Immunosorbent Assay (ELISA) dan
Immunochromatography (ICT)
Peneliti : Citra Dwi Handayani
Pembimbing : Wahdah Norsiah, Tini Elyn Herlina
Acquired Immunodeficiency Syndrome (AIDS) merupakan kumpulan
gejala atau penyakit yang diakibatkan menurunnya kekebalan tubuh akibat infeksi
virus Human Immunodeficiency Virus (HIV). Untuk menegakkan diagnosis HIV
gold standar yang digunakan adalah pemeriksaan Enzyme-Linked Immunosorbent
Assay (ELISA). Pemeriksaan ELISA memiliki waktu yang cukup lama, maka dari
itu untuk uji skrinning menggunakan metode Immunochromathography (ICT),
lalu dilanjutkan dengan uji konfirmasi menggunakan uji ELISA. Tujuan dari
penelitian ini untuk mengetahui apakah ada perbedaan hasil pemeriksaan antibodi
HIV yang diperiksa menggunakan metode ELISA dan Rapid Test. Penelitian ini
menggunakan 30 sampel berupa 15 responden yang terinfeksi HIV dan 15
responden yang tidak terinfeksi HIV. Penelitian ini menggunakan rancangan
penelitian cross sectional study, dengan menggunakan teknik Total Sampling.
Analisis data menggunakan uji Wilcoxon yang dimuat dalam bentuk tabel. Hasil
penelitian didapat pada responden positif HIV dengan jumlah 15 positif pada ICT
dan ELISA, sama halnya pada responden negatif didapat 15 hasil negatif pada
ICT dan ELISA. Hasil analisis menggunakan uji Wilcoxon menunjukkan hasil
1,000 (p > 0,05) yang membuktikan tidak ada perbedaan bermakna dari hasil
tersebut yang dilakukan dengan metode ICT maupun ELISA dan bagi peneliti
selanjutnya lebih memperbanyak jumlah responden dan dilakukan pemeriksaan
berulang pada ICT dengan merk berbeda.
Kata Kunci : HIV/AIDS, ELISA, Immunochromatography (ICT)
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ABSTRACT
Comparison of Test Results for Human Immunodeficiency Virus (HIV)
Antibodies Using Enzyme-Linked Immunosorbent Assay (ELISA) and
Immunochromatography (ICT) Methods
Researcher : Citra Dwi Handayani
Supervisor : Wahdah Norsiah, Tini Elyn Herlina
Acquired Immunodeficiency Syndrome (AIDS) is a collection of
symptoms or diseases caused by decreased immunity due to infection with the
Human Immunodeficiency Virus (HIV). To establish the diagnosis of HIV, the
gold standard used is the Enzyme-Linked Immunosorbent Assay (ELISA). ELISA
examination has a long time, therefore for the screening test using the
Immunochromatography (ICT) method, then proceed with a confirmation test
using the ELISA test. The purpose of this study was to determine whether there
were differences in the results of HIV antibody tests examined using the ELISA
and Rapid Test methods. This study used 30 samples consisting of 15 respondents
who were infected with HIV and 15 respondents who were not infected with HIV.
This study used a cross sectional study design, using the total sampling technique.
Data analysis used the Wilcoxon test which was loaded in tabular form. The
results of the study were obtained from HIV positive respondents with a total of
15 positive results on ICT and ELISA, as well as negative respondents who
obtained 15 negative results on ICT and ELISA. The results of the analysis using
the Wilcoxon test showed a result of 1.000 (p> 0.05) which proved that there was
no significant difference from the results carried out using the ICT and ELISA
methods and for further researchers increased the number of respondents and
repeated examinations were carried out on ICT with different brands.
Keywords: HIV/AIDS, ELISA, Immunochromatography (ICT)
Detail Information
| Item Type | |
|---|---|
| Penulis |
Citra Dwi Handayani - Personal Name
Wahdah Norsiah, M.Si - Personal Name Tini Elyn Herlina - Personal Name |
| Student ID | |
| Dosen Pembimbing | |
| Penguji | |
| Kode Prodi PDDIKTI | |
| Edisi | |
| Departement | |
| Kontributor | |
| Bahasa |
Indonesia
|
| Penerbit | Jurusan Analis Kesehatan : Banjarbaru., 2023 |
| Edisi | |
| Subyek | |
| No Panggil |
009 SKP/ TLM 2023
|
| Copyright | |
| Doi |