Muhammad Ihza Lisan Shidqi - Pengarang
Yayuk Kustiningsih - Dosen Pembimbing 1
Ahmad Muhlisin - Dosen Pembimbing 2

ABSTRAK

PENGARUH EKSTRAK TUMBUHAN KELAKAI (Stenochlaena palustris (Burm. F.) Bedd.) TERHADAP KADAR HEMOGLOBIN PADA MENCIT (Mus musculus)

Penulis : Muhammad Ihza Lisan Shidqi

Pembimbing : Yayuk Kustiningsih, Ahmad Muhlisin

Zat besi dalam tubuh merupakan mineral yang jumlahnya harus tetap tercukupi. Sekitar 80% besi terdapat pada hemoglobin yang berfungsi untuk mengangkut oksigen dari paru-paru ke seluruh tubuh dan membawa kembali karbondioksida dari jaringan ke paru-paru. Di Kalimantan Selatan, tumbuhan kelakai digunakan sebagai bahan makanan dan mengobati penyakit. Kelakai mengandung senyawa seperti flavonoid, alkoloid, dan mineral Fe yang dilaporkan banyak memiliki efek biologis, termasuk aktivitas anti-anemia. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak kelakai terhadap kadar hemoglobin pada mencit. Penelitian ini merupakan studi eksperimental semu dengan PretestPosttest With Control Group Design. Kelompok perlakuan dibagi menjadi 5 kelompok yaitu kelompok yang mendapatkan ekstrak kelakai 2%, 4%, 6%, 8%, dan 10%. Kelompok kontrol tidak mendapatkan ekstrak kelakai. Perlakuan diberikan selama 7 hari setelah mencit diadaptasi. Sampel darah diambil sebelum dan sesudah perlakuan ekstrak kelakai. Kadar hemoglobin diukur dengan Hematology Analyzer. Data dianalisa dengan Uji Repeated Measures ANOVA, dengan tingkat kepercayaan 95%. Terdapat perbedaan bermakna kadar hemoglobin mencit sebelum dan sesudah perlakuan, nilai p=0.0 (p<0.05). Kemudian data dianalisa dengan Uji One Way ANOVA, dengan tingkat kepercayaan 95%. Terdapat perbedaan bermakna antar kelompok perlakuan, nilai p=0.011 (p<0.05). Ekstrak kelakai berpengaruh terhadap kadar hemoglobin mencit pada kelompok 8% (p=0.003) dan kelompok 10% (p=0.031).

Kata Kunci : Hemoglobin, Zat Besi, Kelakai

ABSTRACT

EFFECT OF KELAKAI PLANT EXTRACTS (Stenochlaena palustris (Burm. F.) Bedd.) ON HEMOGLOBIN LEVELS IN MICE (Mus musculus)

Author: Muhammad Ihza Lisan Shidqi

Advisor: Yayuk Kustiningsih, Ahmad Muhlisin

Fe in the body is a mineral whose amount must remain sufficient. About 80% of Fe is found in hemoglobin which functions to carry oxygen from the lungs throughout the body and to bring back carbon dioxide from tissues to the lungs. In South Kalimantan, kelakai plants are used as food and treat diseases. Kelakai contains compounds such as flavonoids, alkoloid, and Fe minerals which are reported to have many biological effects, including anti-anemia activity. The purpose of this study was to determine the effect of kelakai extract on hemoglobin levels in mice. This research is a quasi-experimental study with Pretest-Posttest With Control Group Design. The treatment group was divided into 5 groups: the group that received extracts of kelakai 2%, 4%, 6%, 8%, and 10%. The control group did not get any extracts. The treatment was given for 7 days after the mice were adapted. Blood samples are taken before treatment and after treatment kelakai extract. Hemoglobin levels are measured by Hematology Analyzer. Data were analyzed by the Repeated Measures ANOVA Test, with a confidence level of 95%. There was a significant difference in the hemoglobin level of mice before and after treatment, p value = 0.0 (p <0.05). Then the data were analyzed with the One Way ANOVA Test, with a confidence level of 95%. There were significant differences between treatment groups, p value = 0.011 (p <0.05). Kelakai extract affected mice hemoglobin levels in the 8% group (p = 0.003) and the 10% group (p = 0.031).

Keywords : Hemoglobin, Fe, Kelakai

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