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PERBANDINGAN HASIL PEMERIKSAAN ANTIBODI COVID-19 DENGAN METODE ELISA DAN ICT
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ABSTRAK
PERBANDINGAN HASIL PEMERIKSAAN ANTIBODI COVID-19
DENGAN METODE ELISA DAN ICT
Peneliti : Nisrina Khuzaimah
Pembimbing : H. Haitami, Wahdah Norsiah
Pandemi Covid-19 (Coronavirus disease-19) yang dideklarasikan oleh WHO
pada 11 Maret 2020 sebagai keadaan darurat kesehatan global yang telah
mengakibatkan jutaan kasus korban jiwa memicu ilmuwan dunia untuk
menghentikan pandemi virus Covid-19. Cara yang dinilai paling efektif dan
efesien adalah dengan vaksinasi. Vaksinasi dilakukan agar tubuh membentuk
antibodi sebagai upaya kekebalan tubuh. Perlu adanya test serologis untuk
memonitoring respon imun setelah vaksinasi Covid-19. Tujuan penelitian ini
untuk mengetahui perbandingan hasil pemeriksaan Antibodi Covid-19 dengan
metode ELISA (Enzyme-Linked Immunoabsorbent Assay) dan ICT
(Immunochromatography Test). Penelitian ini bersifat survei analitik dengan
rancangan penelitian cross sectional. Populasi pada penelitian ini adalah
mahasiswa semester VII Jurusan Analis Kesehatan Poltekkes Kemenkes
Banjarmasin yang sudah melakukan vaksin dosis 2 dan 3. Sampel diambil dengan
teknik purposive sampling. Antibodi Covid-19 diuji dengan alat ELISA dan
ICT,serta kuesioner digunakan untuk mengumpulkan data penelitian. Hasil
peneltian menunjukkan pada metode ICT terdapat 26 orang (84%) responden
dinyatakan reaktid dan 4 orang ( 16%) responden dinyatakan non reaktif,
sedangkan metode ELISA terdapat 20 orang (68%) dengan kategori respon imun
kuat (>200 IU/mL) 5 orang (16%) dengan kategori respon imun sedang (100-200
IU/mL), dan 5 orang (16%) dengan kategori respon imun lemah (< 100 IU/mL).
Data diuji analisis dengan uji Wilcoxon dengan nilai p-value 0,083 sehingga
lebih besar dari taraf kemaknaan 0,05 yang artinya tidak ada perbedaan yang
bermakna antara metode ICT dan ELISA. Saran bagi peneliti selanjutnya untuk
melakukan penelitian dengan jumlah dan kriteria responden yang lebih luas ,
Kata kunci : Antibodi Covid-19, Vaksinasi, Immunochromatography Test,
Enzyme-Linked Immunoabsorbent Assay
ix
ABSTRACT
COMPARISON OF ASSESSMENT RESULTS FOR COVID-19
ANTIBODY WITH ELISA METHOD AND ICT
Researcher: Nisrina Khuzaimah
Supervisor : H. Haitami, Wahdah Norsiah
The Covid-19 pandemic (Coronavirus disease-19) which was declared by
WHO as a global health emergency on March 11 2020 which has resulted in
millions of cases of fatalities has prompted world scientists to stop the Covid-
19 virus pandemic. The way that is considered the most effective and efficient
is by vaccination. Vaccination is done so that the body forms antibodies as an
immune effort. There is a need for a serological test to monitor the immune
response after the Covid-19 vaccination. The purpose of this study was to
compare the results of examining Covid-19 antibodies using the ELISA
(Enzyme-Linked Immunoabsorbent Assay) and ICT (Immunochromatography
Test) methods. This research is an analytical survey with a research design
cross sectional. The population in this study were students in semester VII of
the Department of Health Analyst, Poltekkes, Ministry of Health, Banjarmasin,
who had administered vaccine doses 2 and 3. Samples were taken using the
purposive sampling. Covid-19 antibodies were tested using ELISA and ICT
tools, and questionnaires were used to collect research data. The results showed
that in the ICT method there were 26 people (84%) who were declared reactive
and 4 people (16%) who were declared non-reactive, while in the ELISA
method there were 20 people (68%) with a strong immune response (> 200
IU/mL) 5 people (16 %) with moderate immune response (100-200 IU/mL),
and 5 people (16%) with weak immune response (< 100 IU/mL). The data were
tested for analysis using the Wilcoxon test with a p-value of 0.083 so it is
bigger than the significance level of 0.05, which means that there is a not
significant difference between the ICT and ELISA methods. Suggestions for
future researchers to conduct research with a wider number and criteria of
respondents
Keywords: Covid-19 Antibody, Vaccination, Immunochromatography Test,
Enzyme-Linked Immunoabsorbent Assay
Detail Information
| Item Type | |
|---|---|
| Penulis |
Nisrina Khuzaimah - Personal Name
H. Haitami, M.Sc - Personal Name Wahdah Norsiah, M.Si - Personal Name |
| Student ID | |
| Dosen Pembimbing | |
| Penguji | |
| Kode Prodi PDDIKTI | |
| Edisi | |
| Departement | |
| Kontributor | |
| Bahasa |
Indonesia
|
| Penerbit | Jurusan Analis Kesehatan : Banjarbaru., 2023 |
| Edisi | |
| Subyek | |
| No Panggil |
035 SKP/ TLM 2023
|
| Copyright | |
| Doi |