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Gambaran Kualitas Reagen Malachite Green Dan Methylene Blue Pada Pemeriksaan Telur Cacing Dalam Feses Metode Kato-Katz
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ABSTRAK
Gambaran Kualitas Reagen Malachite Green Dan Methylene Blue Pada
Pemeriksaan Telur Cacing Dalam Feses Metode Kato-Katz
Peneliti : Siti Hamdiyyah
Pembimbing : Rifqoh, Jujuk Anton Cahyono
Kecacingan merupakan salah satu penyakit di Indonesia yang sampai saat
ini masih sangat tinggi. Metode pemeriksaan kecacingan salah satunya adalah
metode kato-katz, reagen warna yang digunakan dalam pemeriksaan mikroskopis
metode Kato-Katz yaitu malacite green dan methylene blue yang dilarutkan
dengan aquades dan glycerol. Berfungsi sebagai larutan kato atau reagen, dengan
perendaman selama 24 jam. Tujuan penelitian ini adalah mengetahui kualitas
penyerapan warna selotif dan mengetahui kualitas warna latar belakang
mikroskopis dengan reagen malachite green dan methylene blue. Jenis penelitian
ini menggunakan Quasy experiment, dengan rancangan One group design,
Penelitian ini menggunakan variasi waktu perendaman 1, 6, 12, 18 dan 24 jam.
Sampel feses positif didapatkan dari Laboratorium Kesehatan Provinsi
Kalimantan Selatan. Berdasarkan hasil pemeriksaan diketahui kualitas penyerapan
warna malachite green pada selotif dengan variasi waktu 1, 6, 12, 18 dan 24 jam
didapatkan warna light sea green, sedangkan kualitas penyerapan warna
methylene blue pada selotif dengan variasi waktu 1, 6, 12, 18 dan 24 jam
didapatkan warna cornflower blue. Hasil kualitas warna latar belakang
pemeriksaan di mikroskop dengan reagen malachite green pada variasi waktu 1,
6, 12, 18 dan 24 jam didapatkan warna light sea green, sedangkan reagen
methylene blue pada variasi waktu 1, 6, 12, 18 dan 24 jam didapatkan warna light
cyan. Kesimpulan penelitian ini kualitas penyerapan warna dan kualitas warna
latar belakang adalah reagen malachite green yang menghasilkan warna lebih baik
dibandingkan reagen methylene blue. Bagi peneliti selanjutnya disarankan agar
menambah variabel penelitian seperti kualitas pewarnaan reagen malachite green
dan methylene blue pada warna telur cacing di mikroskop.
Kata Kunci : Pemeriksaan Mikroskopis, Kato- Katz, Malachite green, Methylene
blue
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ABSTRACT
Description of the Quality Malachite Green and Methylene Blue Reagents in
the Examination of Worm Eggs in Feces Kato-Katz Method
Author : Siti Hamdiyyah
Advisor : Rifqoh, Jujuk Anton Cahyono
Worms is one of the diseases in Indonesia which is still very high. One of
the worms examination methods is the kato-katz method, the color reagents used
in the microscopic examination of the Kato-Katz method are malacite green and
methylene blue dissolved in aquades and glycerol. Serves as a cathode solution or
reagent, with immersion for 24 hours. The purpose of this study was to determine
the quality of the color absorption of the adhesive tape and to determine the
quality of the microscopic background color with malachite green and methylene
blue reagents. This type of research uses a quasy experiment, with a One group
design. This study uses a variation of immersion time of 1, 6, 12, 18 and 24 hours.
Positive stool samples were obtained from the Health Laboratory of South
Kalimantan Province. Based on the results of the examination, it is known that the
quality of absorption of malachite green color on tape with time variations of 1, 6,
12, 18 and 24 hours obtained light sea green color, while the quality of absorption
of methylene blue color on tape with time variations of 1, 6, 12, 18 and 24 the
clock got cornflower blue color. The results of the quality of the background color
examination in a microscope with malachite green reagent at time variations of 1,
6, 12, 18 and 24 hours obtained light sea green color, while methylene blue
reagent at time variations of 1, 6, 12, 18 and 24 hours obtained color light cyan.
The conclusion of this study is that the quality of color absorption and the quality
of the background color is malachite green reagent which produces better color
than methylene blue reagent. For further researchers, it is recommended to add
research variables such as the quality of the staining of malachite green and
methylene blue reagents on the color of worm eggs in the microscope.
Keywords : Microscopic Examination, Kato-Katz, Malachite green, Methylene
blue
Detail Information
| Item Type | |
|---|---|
| Penulis |
Siti Hamdiyyah - Personal Name
Rifqoh, M.Sc - Personal Name Jujuk Anton Cahyono, M.Sc - Personal Name Tini Elyn Herlina, MM - Personal Name |
| Student ID | |
| Dosen Pembimbing | |
| Penguji | |
| Kode Prodi PDDIKTI | |
| Edisi | |
| Departement | |
| Kontributor | |
| Bahasa |
Indonesia
|
| Penerbit | Jurusan Analis Kesehatan : Banjarbaru., 2022 |
| Edisi | |
| Subyek | |
| No Panggil |
09/KTI-ANK-2022
|
| Copyright | |
| Doi |