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Perbandingan Hasil Metode ICT dan ELISA untuk Deteksi Anti-HBs pada Tenaga Kesehatan
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ABSTRAK
Perbandingan Hasil Metode ICT dan ELISA untuk Deteksi Anti-HBs pada
Tenaga Kesehatan
Peneliti : Sahri Rahman
Pembimbing : Wahdah Norsiah, Tini Elyn Herlina
Berdasarkan Peraturan Menteri Kesehatan Republik Indonesia Nomor 53
Tahun 2015 petugas kesehatan merupakan kelompok berisiko tinggi terinfeksi Hepatitis
B dan menjadi masalah yang perlu mendapat perhatian, dan vaksinasi cara paling efektif
untuk mengurangi faktor risiko tersebut sehingga untuk mengetahui kadar antibodi antiHBs pada tenaga kesehatan perlu dilakukan pemeriksaan anti-HBs. Tujuan penelitian
ini untuk mengetahui perbandingan hasil antara metode ICT dengan ELISA dalam
pemeriksaan anti-HBs pada tenaga kesehatan. Penelitian ini bersifat deskriptif dengan
rancangan cross-sectional. Populasi pada penelitian ini adalah tenaga kesehatan yang
sudah divaksin Hepatitis B di Puskesmas Wilayah Kabupaten Banjar dan 25 tenaga
kesehatan digunakan sebagai sampel penelitian. Sampel diambil dengan teknik
purposive sampling. Antibodi anti-HBs dalam serum diukur menggunakan
Immunochromatography Test (ICT) dan Enzyme-Linked Immunosorbent Asay (ELISA).
Hasil penelitian menunjukkan pada metode ICT terdapat 19 orang (76%) responden
dinyatakan reaktif dan 6 orang (24%) responden dinyatakan non reaktif, sedangkan
metode ELISA terdapat 15 orang (60%) responden dinyatakan respon imun kuat ( > 100
mIU/mL ) dan 10 orang (40%) responden dinyatakan respon imun lemah ( 10 – 100
mIU/mL ). Data dianalisis dengan uji statistik Chi Square dengan nilai p-value yang
didapat sebesar 0,225 sehingga lebih besar dari taraf kemaknaan 0,05, maka dapat
disimpulkan bahwa tidak terdapat perbedaan hasil yang signifikan antara metode ICT
dan ELISA . Saran kepada petugas kesehatan yang memiliki titer antibodi yang rendah
(10-100 mIU/mL) harus divaksinasi ulang untuk meningkatkan titer anti-HBs, dan bagi
peneliti selanjutnya lebih memperbanyak jumlah responden dengan subjek yang
berbeda.
Kata kunci : Anti-HBs, Tenaga Kesehatan, Immunochromatography Test, EnzymeLinked Immunosorbent Asay
ix
ABSTRACT
Comparison The Results of ICT and ELISA Method for anti-HBs Detection in
Health Workers
Researcher: Sahri Rahman
Supervisor: Wahdah Norsiah, Tini Elyn Herlina
According to Regulation No. 53 of 2015 of the Minister of Health of the Republic of
Indonesian, a high risk of HBV infection in health workers is a problem that requires
attention, and vaccination is effective way to reducing these risk factors so that it is
necessary to determine the level of anti-HBs antibodies in health workers anti-HBs test.
The purpose of this study was to compare the results between the ICT method and
ELISA in the examination of anti-HBs in health workers. This research is descriptive
with a cross-sectional design. The population in this study were health workers who
had been vaccinated against Hepatitis B at the Banjar District Health Center and 25
health workers were used as research samples. Samples were taken by the purposive
sampling technique. Anti-HBs antibody in serum were measured using
Immunochromatography Test (ICT) and Enzyme-Linked Immunosorbent Assay
(ELISA). The results of the study showed that in the ICT method there were 19 (76%)
respondents declared reactive and 6 (24%) respondents declared non-reactive, while the
ELISA method contained 15 (60%) respondents who stated strong immune response (>
100 mIU /mL) and 10 people (40%) of the respondents stated that the immune response
was weak (10-100 mIU/mL). Data analysis with Chi Square statistical test with p-value
obtained is 0.225 so it is greater than the significance level of 0.05, conclusion that there
is no significant difference in results between the ICT method and ELISA. Suggestions
to health workers who have low antibody titers (10-100 mIU/mL) should be revaccinated to increase anti-HBs titers, and for further researchers to more respondents
with different subjects.
Keywords: Anti-HBs, Health Workers, Immunochromatography Test, Enzyme-Linked
Immunosorbent Asay
Detail Information
| Item Type | |
|---|---|
| Penulis |
Sahri Rahman - Personal Name
Wahdah Norsiah, M.Si - Personal Name Tini Elyn Herlina, MM - Personal Name Dinna Rakhmina, S.Si., M.Sc - Personal Name |
| Student ID | |
| Dosen Pembimbing | |
| Penguji | |
| Kode Prodi PDDIKTI | |
| Edisi | |
| Departement | |
| Kontributor | |
| Bahasa |
Indonesia
|
| Penerbit | Jurusan Analis Kesehatan : Banjarbaru., 2022 |
| Edisi | |
| Subyek | |
| No Panggil |
024/ANK-SKP-2022
|
| Copyright | |
| Doi |