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Perbedaan Morfologi Sel Eritrosit dan Sel Leukosit Metode Wright Stain Menggunakan Buffer Fosfat pH 6,8 dan Air Mineral Bermerk Lokal
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ABSTRAK
Perbedaan Morfologi Sel Eritrosit dan Sel Leukosit Metode Wright Stain
Menggunakan Buffer Fosfat pH 6,8 dan Air Mineral Bermerk Lokal
Peneliti : Ghina Nu’ma Nabiela
Pembimbing : H. Ahmad Muhlisin ; Neni Oktiyani
Metode pewarnaan Sediaan Apusan Darah Tepi (SADT) yang dianjurkan
oleh The Internasional Council For Standardization in Haematology (ICSH) salah
satunya adalah Wright Stain yang digunakan untuk pengamatan sel dan komponen
sehingga dapat melihat morfologi sel darah. Ketepatan Power of Hydrogen (pH)
buffer sebagai larutan pengencer Wright Stain harus diperhatikan untuk hasil
pewarnaan yang baik. Larutan buffer fosfat pH 6,8 mudah didapatkan, namun
harganya cukup mahal, sehingga air mineral digunakan sebagai larutan alternatif
yang bisa digunakan sebagai larutan pengencer yang lebih murah dan mudah untuk
diperoleh. Penelitian ini bertujuan untuk mengetahui perbedaan hasil morfologi sel
eritrosit dan sel leukosit Metode Wright Stain menggunakan Buffer Fosfat pH 6,8
dan Air Mineral Bermerk Lokal. Jenis penelitian observasional laboratorium
dengan rancangan comparative study. Menggunakan 6 jenis air mineral bermerk
lokal yang diproduksi wilayah Kalimantan Selatan dan 3 level darah kontrol yaitu
high, normal, dan low. Pengulangan yang dilakukan 5 kali dalam satu jenis darah
kontrol. Hasil penelitian morfologi sel eritrosit dan sel leukosit pada darah kontrol
high, normal dan low menggunakan buffer fosfat pH 6,8 dan air mineral bermerk
lokal A, B, C, D, E, dan F memiliki hasil pewarnaan yang baik. Berdasarkan uji
Friedman dari ketiga jenis darah kontrol mendapatkan nilai signifikansi >0,05
berarti tidak terdapat perbedaan bermakna antara penggunaan buffer fosfat pH 6,8
dan air mineral bermerk lokal A, B, C, D, E, dan F. Sehingga dapat disimpulkan air
mineral bermerk lokal A, B, C, D, E, dan F dapat digunakan sebagai larutan
pengencer alternatif pengganti buffer fosfat pH 6,8.
Kata kunci : Morfologi Sel Eritrosit, Morfologi Sel Leukosit, Buffer Fosfat, Air
Mineral Bermerk Lokal
ABSTRACT
The Differences in Morphology of Erythrocyte and Leukocyte Cells Wright
Stain Method Using Phosphate Buffer pH 6.8 and Local Branded Mineral
Water
Author : Ghina Nu'ma Nabiela
Supervisor : H. Ahmad Muhlisin ; Neni Oktiyani
One of the staining methods for Peripheral Blood Smear (SADT)
recommended by The International Council for Standardization in Haematology
(ICSH) is the Wright Stain which is used for observing cells and components so
that they can see the morphology of blood cells. The accuracy of the Power of
Hydrogen (pH) buffer as a diluent for Wright Stain must be considered for good
staining results. Phosphate buffer solution pH 6.8 is easy to obtain, but the price is
quite expensive, so mineral water is used as an alternative solution that can be used
as a diluent solution that is cheaper and easier to obtain. Aims to determine
differences in morphological results of erythrocyte and leukocyte cells. Wright
Stain method using Phosphate Buffer pH 6.8 and Local Branded Mineral Water.
This type of research is an observational laboratory with a comparative study
design. 6 types of local branded mineral water were used, produced in the South
Kalimantan region and 3 control blood levels, namely high, normal, and low.
Repetition was carried out 5 times in one type of control blood. The results of the
study of morphology of erythrocytes and leukocytes in high, normal and low control
blood using phosphate buffer pH 6.8 and local branded mineral water A, B, C, D,
E, and F had good staining results. Based on the Friedman test, the three control
blood types obtained a significance value > 0.05 meaning there was no significant
difference between the use of phosphate buffer pH 6.8 and local branded mineral
water A, B, C, D, E, and F. So it can be concluded that water local branded minerals
A, B, C, D, E, and F can be used as an alternative diluent to replace phosphate buffer
pH 6.8.
Keywords : Erythrocyte Cell Morphology, Leukocyte Cell Morphology, Phosphate
Buffer, Local Branded Mineral Water
Detail Information
| Item Type | |
|---|---|
| Penulis |
Ghina Nu’ma Nabiela - Personal Name
H. Ahmad Muhlisin, M.Kes - Personal Name Neni Oktiyani, M.Sc - Personal Name Wahdah Norsiah, M.Si - Personal Name |
| Student ID | |
| Dosen Pembimbing | |
| Penguji | |
| Kode Prodi PDDIKTI | |
| Edisi | |
| Departement | |
| Kontributor | |
| Bahasa |
Indonesia
|
| Penerbit | Jurusan Analis Kesehatan : Banjarbaru., 2022 |
| Edisi | |
| Subyek | |
| No Panggil |
ANK/ 04 SKP 2022
|
| Copyright | |
| Doi |