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Jujuk Anton Cahyono, S.Si.,M.Sc Dosen Pembimbing 2 Afrelicia Praoenitiyas Sutarto Pengarang Ahmad Muhlisin, Dosen Pembimbing 1

ABSTRAK PERBEDAAN JUMLAH LEUKOSIT MENGGUNAKAN LARUTAN TURK DAN LARUTAN TURK YANG DIMODIFIKASI DENGAN PERASAN AIR LEMON (Citrus limon) PADA VARIASI WAKTU PENYIMPANAN Penulis : Afrelicia Praoenitiyas Sutarto Pembimbing : Ahmad Muhlisin, Jujuk Anton Cahyono Perhitungan jumlah leukosit metode manual memerlukan reagen larutan Turk. Komposisi larutan Turk standar adalah asam asetat glasial 2%, gentian violet 1% dan aquadest. Jeruk lemon (Citrus limon) memiliki kandungan asam sitrat. Asam Sitrat pada jeruk lemon merupakan asam lemah yang mana sama dengan Asam asetat. Penelitian ini bertujuan untuk mengetahui perbedaan jumlah leukosit dengan menggunakan larutan Turk dan larutan Turk yang dimodifikasi dengan perasan air lemon yang segera diperiksa, disimpan 1 minggu, 2 minggu, dan 3 minggu dengan konsentrasi 80%. Jenis penelitian yang digunakan adalah experiment dengan rancangan posttest only control group design. Analisis data menggunakan uji T-test Dependent, rata-rata jumlah leukosit larutan Turk segera, disimpan 1 minggu, 2 minggu, dan 3 minggu yaitu 6.713 sel/mm3 , 6.238 sel/mm3 , 6.175 sel/mm3 ,dan 5.975 sel/mm3 . Rata-rata jumlah leukosit larutan Turk modifikasi perasan air lemon segera, disimpan 1 minggu, 2 minggu, dan 3 minggu yaitu 6.613 sel/mm3 , 6.125 sel/mm3 , 5.850 sel/mm3 ,dan 5.625 sel/mm3 . Pada uji statistik larutan Turk dan larutan Turk modifikasi perasan air lemon segera didapatkan hasil 0,116 dan pada 1 minggu didapatkan hasil 0,078 maka menunjukan tidak terdapat perbedaan. Terdapat perbedaan antara larutan Turk dan larutan Turk modifikasi perasan air lemon disimpan 2 minggu dan 3 minggu karena nilai signifikansi <0,05. Kesimpulan penelitian ini tidak terdapat perbedaan jumlah leukosit pada larutan Turk dan larutan Turk yang dimodifikasi perasan air lemon segera diperiksa dan disimpan 1 minggu. Saran penelitian ini untuk peneliti selanjutnya dapat melakukan penelitian dengan jenis jeruk atau buah yang berbeda sebagai pengganti asam asetat dengan variasi waktu penyimpanan. Kata Kunci: Leukosit, Lemon, Variasi waktu penyimpanan ABSTRACT DIFFERENCE IN LEUKOSIT AMOUNT USING TURK SOLUTION AND TURK SOLUTION MODIFIED WITH LEMON (Citrus limon) WATER PRESSURE ON VARIATION OF STORAGE TIME Author : Afrelicia Praoenitiyas Sutarto Supervisor : Ahmad Muhlisin, Jujuk Anton Cahyono Manual method leukocyte count requires Turk's solution reagent. The composition of the standard Turk's solution is 2% glacial acetic acid, 1% gentian violet and distilled water. Lemon (Citrus limon) has citric acid content. Citric acid in lemon is a weak acid which is the same as acetic acid. This study aims to determine the difference in leukocyte counts using Turk's solution and modified Turk's solution with lemon juice that is immediately examined, stored for 1 week, 2 weeks, and 3 weeks with a concentration of 80%. The type of research used is experiment with posttest only control group design. Data analysis using Dependent T-test, the average leukocyte count of Turk solution immediately, stored 1 week, 2 weeks, and 3 weeks are 6,713 cells/mm3, 6,238 cells/mm3, 6,175 cells/mm3, and 5,975 cells/mm3. The average leukocyte counts of the modified Turk solution of lemon juice immediately, stored for 1 week, 2 weeks, and 3 weeks were 6,613 cells/mm3, 6,125 cells/mm3, 5,850 cells/mm3, and 5,625 cells/mm3. In the statistical test of Turk's solution and Turk's solution modified lemon juice immediately obtained the result of 0.116 and at 1 week obtained the result of 0.078, it shows there is no difference. There is a difference between Turk's solution and modified Turk's solution of lemon juice stored 2 weeks and 3 weeks because the significance value <0.05. The conclusion of this study is that there is no difference in the number of leukocytes in the Turk solution and the modified Turk solution of lemon juice immediately examined and stored for 1 week. The suggestion of this study for further researchers can conduct research with different types of citrus or fruit as a substitute for acetic acid with variations in storage time. Keywords: Leukocytes, Lemon, Storage time variation