Dra. Anny Thuraidah, Apt.,MS. Dosen Pembimbing 2 Wahdah Norsiah, M.Si Dosen Pembimbing 1 Nadia Ulfahma Pengarang

ABSTRAK PERBANDINGAN HITUNG JUMLAH LEUKOSIT MENGGUNAKAN LARUTAN TURK DENGAN LARUTAN MODIFIKASI TURK DARI PERASAN JERUK NIPIS (Citrus aurantifolia ) DAN EKSTRAK UBI JALAR UNGU (Ipomoea batatas L) Penulis: Nadia Ulfahma Pembimbing: Wahdah Norsiah, Anny Thuraidah Pemeriksaan hitung jumlah leukosit metode manual memerlukan reagen Larutan Turk. Larutan Turk terdiri dari larutan asam asetat glasial 2% dan gentian violet 1%. Pada laboratorium klinik sederhana, ketersediaan reagen Turk untuk menghitung jumlah leukosit seringkali tidak tersedia atau tersedia tetapi kadaluarsa. Selain itu Gentian Violet 0,24-5 mg/L dalam 48 jam memiliki sifat sangat toksik. Penelitian ini bertujuan untuk mengetahui perbandingan hitung jumlah leukosit menggunakan larutan Turk dengan larutan modifikasi Turk perasan jeruk nipis (Citrus aurantifolia) dan ekstrak ubi jalar ungu (Ipomoea batatas L). Penelitian ini bersifat true experiment dengan rancangan posttest only control group design. Sampel penelitian menggunakan darah kontrol normal. Rata-rata hasil pemeriksaan hitung jumlah leukosit menggunakan larutan Turk adalah 7.390 sel/mm3. Hasil rerata hitung jumlah leukosit menggunakan modifikasi pada jeruk nipis 2% dan gentian violet 1% yaitu 7.080 sel/mm3, jeruk nipis 3% dan gentian violet 1% yaitu 6.870 sel/mm3, ubi jalar ungu 1% dan asam asetat glasial yaitu 6.810 sel/mm3, jeruk nipis 2% dan ubi jalar ungu 1% yaitu 7.100 sel/mm3, serta jeruk nipis 3% dan ubi jalar ungu 1% yaitu 6.840 sel/mm3. Melalui uji statistik Reapeted ANOVA didapatkan nilai sig 0,646 (sig>α). Kesimpulan: tidak terdapat perbedaan bermakna antara Turk dengan Modifikasi Turk. Saran: Penelitian ini diharapkan dapat menambah pengembangan ilmu dibidang Hematologi serta acuan dalam pemanfaatan bahan alami sebagai pengganti Turk pada pemeriksaan Hitung Jumlah Leukosit. Kata Kunci: Leukosit, Turk, Jeruk Nipis, Ubi Jalar Ungu ix ABSTRACK COMPARISON OF LEUCOCYTE NUMBER COUNT USING TURK'S SOLUTION WITH TURK'S MODIFIED SOLUTION FROM LIME JUICE (Citrus aurantifolia) AND PURPLE SWEET POTATO EXTRACT (Ipomoea batatas L) Author: Nadia Ulfahma Supervisor: Wahdah Norsiah,Anny Thuraidah Manual leukocyte count examination requires Turk's solution reagent. Turk's solution consists of acetic acid glasial 2% and gentian violet 1% solution. In simple clinical laboratories, the availability of Turk's reagent for leukocyte counts is often unavailable or available but expired. In addition, Gentian Violet 0.24-5 mg/L in 48 hours is highly toxic. This study aims to determine the comparison of leukocyte counts using Turk's solution with a modified Turk's solution of lime juice (Citrus aurantifolia) and purple sweet potato extract (Ipomoea batatas L). This research is a true experiment with posttest only control group design. The research sample used normal control blood. The average result of the leukocyte count examination using Turk's solution was 7,390 cells/mm3. The average result of leukocyte count using modification in lime 2% and gentian violet 1% is 7,080 cells/mm3, lime 3% and gentian violet 1% is 6,870 cells/mm3, purple sweet potato 1% and acetic acid glasial is 6,810 cells/mm3, lime 2% and purple sweet potato 1% is 7,100 cells/mm3, and lime 3% and purple sweet potato 1% is 6,840 cells/mm3. Through Reapeted ANOVA statistical test, the sig value was 0.646 (sig>α). Conclusion: there is no significant difference between Turk and Modified Turk. Suggestion: This research is expected to add to the development of knowledge in the field of Hematology as well as a template in the use of natural substances as a substitute for Turk in the Leukocyte Count examination. Keywords: Leukocyte, Turk, Lime, Purple Sweet Potato