Dra. Anny Thuraidah, Apt.,MS. Dosen Pembimbing 2 Yayuk Kustiningsih, M.Kes Dosen Pembimbing 1 Prisma Selvia Pengarang

ABSTRAK Pengaruh Variasi Waktu Penundaan Pemeriksaan Terhadap Kadar Elektrolit (Na, K, Ca) pada Sampel Serum di RSUD dr. Doris Sylvanus Peneliti: Prisma Selvia Pembimbing: Yayuk Kustiningsih, Anny Thuraidah Pemeriksaan elektrolit darah merupakan salah satu pemeriksaan laboratorium klinik yang sangat dipengaruhi oleh tahapan pra analitik. Penundaan pemeriksaan adalah salah satu faktor yang dapat mempengaruhi pemeriksaan elektrolit. Tujuan penelitian ini untuk mengetahui pengaruh variasi waktu penundaan pemeriksaan terhadap kadar elektrolit (Na, K, Ca) pada sampel serum di RSUD dr. Doris Sylvanus. Metode penelitian ini menggunakan metode experiment dan menggunakan rancangan post test only with control. Subjek penelitian ini adalah pasien medical check up berjenis kelamin laki-laki yang dinyatakan sehat dan tidak mengidap suatu penyakit. Pemeriksaan sampel menggunakan elektrolit Analyzer GEM 3500 Premier dengan metode ISE (Ion Selective Elektrode). Analisis data menggunakan program komputer dengan Uji Repeated Anova. Hasil penelitian menunjukkan rata-rata kadar natrium 0 jam, 1 jam, 2 jam dan 3 jam berturut-turut adalah 139 mmol/L, 139 mmol/L, 141 mmol/L dan142 mmol/L. Rata-rata kadar kalium 0 jam, 1 jam, 2 jam dan 3 jam berturut- turut adalah 4,2 mmol/L, 4,2 mmol/L, 4,3 mmol/L, dan 4,4 mmol/L. Rata-rata kadar kalsium 0 jam, 1 jam, 2 jam dan 3 jam berturut-turut adalah 1,17 mmo/L, 1,15 mmol/L, 1,13 mmol/L, dan 1,11 mmol/L. Uji Repeated Anova menunjukkan hasil natrium dengan signifikansi 0,000 (< 0,05), kalium dengan signifikansi 0,009 (< 0,05), kalsium dengan signifikansi 0,000 (< 0,05) artinya ada perbedaan yang bermakna dari variasi waktu penundaan pemeriksaan terhadap kadar natrium, kalium dan kalsium dengan variasi waktu 0 jam, 1 jam, 2 jam dan 3 jam. Kesimpulan ada pengaruh variasi waktu penundaan pemeriksaan terhadap kadar natrium, kalium dan kalsium pada sampel serum dengan variasi waktu 0 jam, 1 jam, 2 jam dan 3 jam. Saran penelitian dapat dilakukan penelitian lebih lanjut terkait pengaruh suhu penyimpanan sampel terhadap kadar elektrolit (Na, K, Ca). Kata kunci: Kadar elektrolit (Na, K, Ca), Waktu Penundaan Pemeriksaan ABSTRACT Effect of Time Variation of Electrolyte Examination Delay (Na, K, Ca) on Serum Samples at RSUD dr. Doris Sylvanus Author: Prisma Selvia Advisor: Yayuk Kustiningsih, Anny Thuraidah Blood electrolyte examination is one of the clinical laboratory tests that is strongly influenced by the pre-analytical stage. Examination delay is one of the factors that can affect electrolyte examination. Every 30 minutes after the blood draw, a delay in the examination of the blood sample allows the electrolyte levels in the tube to decrease. The purpose of this study was to determine the effect of variations in test delay time on electrolyte levels (Na, K, Ca) in serum samples at RSUD dr. Doris Sylvanus. This research method uses an experimental method and uses a post test only design with control with purposive sampling techniques. The subjects of this study were male medical check-up patients who were declared healthy and did not suffer from a disease. Sample examination using the GEM 3500 Premier Analyzer electrolyte. Data analysis using a computer program with the Repeated Anova Test. The results showed that the average sodium rate of 0 hours, 1 hour, 2 hours and 3 consecutive hours was 139 mmol/L, 139 mmol/L, 141 mmol/L and 142 mmol/L. The average potassium rate of 0 hours, 1 hour, 2 hours and 3 consecutive hours is 4,2 mmol/L, 4,2 mmol/L, 4,3 mmol/L, and 4.4 mmol/L. The average calcium rate of 0 hours, 1 hour, 2 hours and 3 consecutive hours are 1.17 mmo/L, 1.15 mmol/L, 1.13 mmol/L, and 1.11 mmol/L. Repeated Anova test shows sodium results with sig. 0.000 (< 0.05), potassium with sig. 0.009 (< 0.05), calcium with sig. 0.000 (< 0.05) means that there is a significant difference from the variation in the delay time of examination of sodium, potassium and calcium levels with time variations of 0 hours, 1 hour, 2 hours and 3 hours. Conclusion there is an influence of variations in test delay time on sodium, potassium and calcium levels in serum samples with time variations of 0 hours, 1 hour, 2 hours and 3 hours. Research suggestions can be carried out further research related to the effect of sample storage temperature on electrolyte levels (Na, K, Ca). Keywords: Electrolyte Levels (Sodium, Potassium, Calcium), Examination Delay Time