H. Ahmad Muhlisin Dosen Pembimbing 2 Jujuk Anton Cahyono, S.Si.,M.Sc Dosen Pembimbing 1 Nur Anisa Pengarang

ABSTRAK Perbandingan Nilai Laju Endap Darah (LED) Metode Westergren dengan Perbedaan Konsentrasi Natrium Sitrat Penulis : Nur Anisa Pembimbing : Jujuk Anton Cahyono, Ahmad Muhlisin Pemeriksaan laju endap darah (LED) merupakan pemeriksaan yang mengukur kecepatan pengendapan eritrosit, menggambarkan komposisi plasma, dan mengukur perbandingan antara eritrosit dan plasma. Antikoagulan yang umumnya digunakan pada pemeriksaan LED adalah Natrium Sitrat. Menurut International Commitee for Standardization in Hematology (ICSH) konsentrasi antikoagulan Natrium Sitrat dapat digunakan 2,9-3,9%. Penelitian ini bertujuan untuk mengetahui perbedaan nilai LED metode westergren dengan menggunakan Na. Sitrat 3,2%, Na. Sitrat 3,8 %, dan Na. Sitrat 4,4%. Jenis penelitian ini adalah eksperimen semu dengan rancangan penelitian komparatif. Populasi dalam penelitian ini berjumlah 27 sampel. Nilai LED menggunakan Na. Sitrat 3,2% didapatkan rata-rata 18 mm/jam dengan nilai LED berkisar 4-60 mm/jam, Na. Sitrat 3,8% didapatkan rata-rata 18 mm/jam dengan nilai LED berkisar 3-62 mm/jam, Na. Sitrat 4,4% didapatkan rata-rata 19 mm/jam dengan nilai LED berkisar 5-60 mm/jam. Hasil statistik uji Wilcoxon nilai LED Na. Sitrat 3,2% - Na. Sitrat 3,8% didapatkan nilai sig 0.674, nilai LED Na. Sitrat 3,8% - Na. Sitrat 4,4% didapatkan nilai sig 0.022, nilai LED Na. Sitrat 3,2% - Na. Sitrat 4,4% didapatkan nilai sig 0.031. Hasil yang ditemukan tidak terdapat perbedaan yang bermakna antara nilai LED Na. Sitrat 3,2% dan Na. Sitrat 3,8%, sedangkan terdapat perbedaan yang bermakna Na. Sitrat 3,8% - Na. Sitrat 4,4% dan nilai LED Na. Sitrat 3,2% - Na. Sitrat 4,4%. Saran untuk penelitian lebih lanjut tentang pengaruh Natrium Sitrat 3,2%, 3,8%, dan 4,4% pada morfologi dan ukuran eritrosit terhadap nilai LED. Kata Kunci : Laju Endap Darah (LED), Antikoagulan Natrium Sitrat, Westergren ix ABSTRACT Comparison of Blood Sedimentation Rate (LED) Values of the Westergren Method with Different Sodium Citrate Concentrations Author: Nur Anisa Supervisors: Jujuk Anton Cahyono, Ahmad Muhlisin Erythrocyte sedimentation rate (ESR) examination is an examination that measures the sedimentation rate of erythrocytes, describes the composition of plasma, and measures the ratio between erythrocytes and plasma. The anticoagulant generally used in ESR examinations is sodium citrate. According toInternational Commitee for Standardization in Hematology (ICSH) concentration of the anticoagulant Sodium Citrate can be used at 2.9-3.9%. This study aims to determine the difference in LED values using the Westergren method using Na. Citrate 3.2%, Na. Citrate 3.8%, and Na. Citrate 4.4%. This type of research is a quasi-experiment with a comparative research design. The population in this study consisted of 27 samples. LED values use Na. Citrate 3.2% obtained an average of 18 mm/hour with ESR values ranging from 4-60 mm/hour, Na. Citrate 3.8% obtained an average of 18 mm/hour with ESR values ranging from 3-62 mm/hour, Na. Citrate 4.4% obtained an average of 19 mm/hour with LED values ranging from 5-60 mm/hour. Statistical results of the Wilcoxon test for Na LED values. Citrate 3.2% - Na. Citrate 3.8% obtained a sig value of 0.674, the LED value of Na. Citrate 3.8% - Na. Citrate 4.4% obtained a sig value of 0.022, the LED value of Na. Citrate 3.2% - Na. Citrate 4.4% obtained a sig value of 0.031. The results found that there was no significant difference between the Na LED values. Citrate 3.2% and Na. Citrate was 3.8%, while there was a significant difference in Na. Citrate 3.8% - Na. Citrate 4.4% and LED value Na. Citrate 3.2% - Na. Citrate 4.4%. Suggestions for further research on the effect of 3.2%, 3.8% and 4.4% Sodium Citrate on erythrocyte morphology and size on ESR values. Keywords : Erythrocyte Sedimentation Rate (ESR), Sodium Citrate Anticoagulant, Westergren