Dra. Anny Thuraidah, Apt.,MS. Dosen Pembimbing 2 Arista Triyanti Pengarang Rifqoh, M.Sc Dosen Pembimbing 1 Tini Elyn Herlina, MM Ketua Penguji

ABSTRAK Efektifitas Larvasida Ekstrak Etanol Daun Bangkal Dalam Membunuh Larva Nyamuk Aedes sp. Peneliti : Arista Triyanti Pembimbing : Rifqoh dan Anny Thuraidah Demam berdarah dengue (DBD) adalah penyakit infeksi yang disebabkan oleh virus dengue. Penggunaan larvasida kimiawi abate (themepos) terus-menerus mengakibatkan resistensi larva nyamuk dan pencemaran lingkungan, maka diperlukan larvasida alternatif dari tanaman, Salah satu contoh larvasida alternatif yaitu daun Bangkal (Nauclea subdita). Tujuan dari penelitian ini untuk mengetahui ada tidaknya efektifitas ekstrak etanol daun Bangkal terhadap mortalitas larva nyamuk Aedes sp. Metode penelitian true experiment rancangan penelitian posttest only control group design. Bahan penelitian daun Bangkal dan bahan uji larva Aedes sp Instar III. Ekstrak etanol daun Bangkal diperoleh dengan maserasi selama 3x24 jam. Pengujian dilakukan terhadap 3 kelompok, yaitu kelompok kontrol negatif, kontrol positif temephos 1 ppm dan perlakuan konsentrasi (2%, 4%, 6%, 8% dan 10%). Masing-masing perlakuan dengan 20 larva uji dan 4 kali pengulangan. Kematian larva 100% (20 ekor) setelah 6 jam perlakuan ada pada konsentrasi 10%, 12 jam perlakuan ada pada konsentrasi 6% dan setelah 24 jam perlakuan ada pada konsentrasi 4%. Estimasi LC90 perlakuan 6 jam, 12 jam dan 24 jam terletak pada konsentrasi 9.178%, 4.969% dan 1.185%. Didapat kesimpulan bahwa ekstrak etanol daun Bangkal (Nauclea subdita) efektif dalam membunuh larva Aedes sp. Disarankan pada peneliti selanjutnya melakukan uji pendahuluan untuk menentukan konsentrasi ekstrak etanol daun Bangkal dengan konsentrasi lebih rendah yang efektif dalam membunuh populasi larva uji terhadap spesies yang berbeda dan melakukan penelitian dengan mencoba pelarut dan metode yang berbeda sehingga dapat diketahui hasil ekstraksi yang lebih optimal. Kata Kunci : Ekstrak etanol , Daun Bangkal, Mortalitas, Larva Aedes sp. ABSTRACT Effectiveness of Bangkal Leaf Ethanol Extract Larvicidal in Killing Aedes sp. mosquito larvae Researcher: Arista Triyanti Advisors: Rifqoh and Anny Thuraidah Dengue hemorrhagic fever (DHF) is an infectious disease caused by the dengue virus. The continuous use of the chemical abate (themepos) larvicides has resulted in mosquito larvae resistance and environmental pollution, so alternative larvicides from plants are needed. One example of an alternative larvicide is Bangkal leaves (Nauclea subdita). The purpose of this study was to determine whether there was effectiveness of the ethanol extract of Bangkal leaves on the mortality of Aedes sp. mosquito larvae. The research method is true experiment research design posttest only control group design. Bangkal leaf research material and Aedes sp Instar III larvae test material. The ethanol extract of Bangkal leaves was obtained by maceration for 3x24 hours. Tests were carried out on 3 groups, namely the negative control group, the positive control group of 1 ppm temephos and the concentration treatment (2%, 4%, 6%, 8% and 10%). Each treatment with 20 test larvae and 4 repetitions. Larvae mortality was 100% (20 tails) after 6 hours of treatment at a concentration of 10%, 12 hours of treatment at a concentration of 6% and after 24 hours of treatment at a concentration of 4%. LC90 estimation for 6 hours, 12 hours and 24 hours treatment is located at concentrations of 9.178%, 4.969% and 1.185%. It was concluded that the ethanol extract of Bangkal leaves (Nauclea subdita) was effective in killing Aedes sp. larvae. It is recommended that the next researcher conduct a preliminary test to determine the concentration of the ethanol extract of Bangkal leaves with a lower concentration that is effective in killing the test larvae population of different species and conduct research by trying different solvents and methods so that more optimal extraction results can be identified. Keywords: Ethanol extract, Bangkal leaves, Mortality, Aedes sp.