Wahdah Norsiah, M.Si Dosen Pembimbing 2 Dra. Ratih Dewi Dwiyanti, M. Kes Dosen Pembimbing 1 Aima Insana, S.Si., M.Si Ketua Penguji Yola Febrianti Pengarang

ABSTRAK Perbandingan Hasil Pemeriksaan Mycobacterium tuberculosis Pada Sampel Sputum Segera Dan Tunda 12 Jam Dengan Metode Tes Cepat Molekuler (TCM) Peneliti : Yola Febrianti Pembimbing : Ratih Dewi Dwiyanti, Wahdah Norsiah Tuberkulosis merupakan masalah kesehatan masyarakat yang masih menjadi tantangan secara global dan nasional. Penyakit tuberkulosis disebabkan oleh bakteri Mycobacterium tuberculosis. Untuk menegakkan diagnosa penyakit TB dengan pemeriksaan laboratorium menggunakan metode Tes Cepat Molekuler (TCM) karena metode ini lebih sensitif dan akurat dibandingkan metode konvensional, dan sekaligus dapat mendeteksi sensitivitas Rifampisin. Tujuan penelitian ini untuk mengetahui perbedaan hasil pemeriksaan TB pada metode TCM menggunakan alat GeneXpert terhadap penundaan penanganan sputum pada tersangka tuberkulosis di RSUD Pangeran Jaya Sumitra Kotabaru. Metode penelitian ini adalah Survey Analitik dengan menggunakan rancangan penelitian Cross Sectional Studies dengan membandingkan hasil pemeriksaan Mycobacterium tuberculosis pada sampel sputum yang diperiksa segera dengan sampel sputum yang ditunda selama 12 jam pada suhu ruang dan selanjutnya data di analisis secara statistik menggunakan uji Wilcoxon. Hasil penelitian menunjukkan bahwa dari total 46 sampel sputum yang dilakukan penelitian didapatkan hasil pemeriksaan sputum segera yaitu MTB Not Detected 33 sampel, MTB Detected Very Low 3 sampel, MTB Detected Low 6 sampel, dan MTB Detected Medium 4 sampel. Untuk sampel yang ditunda 12 jam pada suhu ruang didapatkan hasil MTB Not Detected 33 sampel, MTB Detected Very Low 3 sampel, MTB Detected Low 6 sampel, dan MTB Detected Medium 4 sampel. Kesimpulan penelitian ini yaitu tidak terdapat perbedaan hasil pemeriksaan Mycobacterium tuberculosis pada sampel sputum segera dengan yang ditunda 12 jam pada suhu ruang. Saran penelitian ini adalah diperlukan penelitian lebih lanjut mengenai variabel lain yang dapat mempengaruhi hasil pemeriksaan TB metode TCM seperti variasi waktu penundaan, suhu, dan lain sebagainya. Kata Kunci: Mycobacterium tuberculosis, Tes Cepat Molekuler, Sputum viii ABSTRACT Comparison of Mycobacterium tuberculosis Test Result on Sputum Samples Between Immediate and 12 Hours Postponed Samples With The Molecular Rapid Test (TCM) Method Author : Yola Febrianti Advisor : Ratih Dewi Dwiyanti, Wahdah Norsiah Tuberculosis is a public health problem that remains a challenge globally and nationally. Tuberculosis disease caused by the Mycobacterium tuberculosis bacterium. To diagnose TB disease, laboratory examination using the Molecular Rapid Test (TCM) can be the most efficient method because this method is more sensitive and accurate than conventional methods, and at the same time can detect Rifampicin sensitivity. The purpose of this study was to determine the difference in the results of TB examination in the TCM method using the GeneXpert tool against delays in handling sputum toward tuberculosis suspects at Pangeran Jaya Sumitra Kotabaru Hospital. This research method was Analytical Survey using the Cross Sectional Studies research design by comparing the results of the Mycobacterium tuberculosis examination on the sputum sample examined immediately against sputum sample which was delayed for 12 hours at room temperature and then the data was analyzed statistically using the Wilcoxon test. The results showed that from a total of 46 sputum samples observed, the results of the sputum examination were immediately obtained, namely MTB Not Detected 33 samples, MTB Detected Very Low 3 samples, MTB Detected Low 6 samples, and MTB Detected Medium 4 samples. For samples that were delayed by 12 hours at room temperature, the results of MTB Not Detected 33 samples, MTB Detected Very Low 3 samples, MTB Detected Low 6 samples, and MTB Detected Medium 4 samples were obtained. The conclusion of this study is that there is no difference in the results of the Mycobacterium tuberculosis test on the sputum sample immediately with those postponed by 12 hours at room temperature. The suggestion of this research is that further research is needed regarding other variables that can affect the results of the TB examination of the TCM method such as variations in delay of time, temperature, and so on. Keywords : Mycobacterium tuberculosis, Molecular Rapid Test, Sputum