H. Haitami, M.Sc Ketua Penguji Neni Oktiyani, M.Sc Dosen Pembimbing 1 H. Ahmad Muhlisin, M.Kes Dosen Pembimbing 2 Nani Jaini Pengarang

ABSTRAK Pengaruh Waktu Inkubasi Antibodi Primer Helicobacter pylori Terhadap Ketajaman Warna Reaksi Pada Pemeriksaan Autostaining Imunohistokimia Biopsi Gaster Pasien Gastritis Peneliti : Nani Jaini Pembimbing : Neni Oktiyani, Ahmad Muhlisin Inkubasi antibodi primer merupakan protokol yang harus dikerjakan pada proses Imunohistokimia inkubasi yang mengalami masalah menyebabkan buruknya intensitas pewarnaan. Protokol pengecatan Imunohistokimia pada biopsi gaster pasien gastritis menggunakan alat Autostaining Roche Tissue Diagnostics Ventana BenchMark XT tidak menyertakan waktu inkubasi dari antibodi primer Helicobacter pylori. Uji pendahuluan dengan waktu inkubasi 16 menit didapatkan warna reaksi ideal dan waktu inkubasi 20 menit warna reaksi overstaining, sedangkan waktu inkubasi di bawah 16 menit belum diketahui warna reaksinya. Tujuan penelitian ini adalah mengetahui ketajaman warna reaksi pemeriksaan Autostaining Imunohistokimia biopsi gaster pasien gastritis Helicobacter pylori dengan waktu inkubasi antibodi primer 8 menit, 12 menit dan 16 menit. Penelitian dilakukan di Instalasi Laboratorium Patologi Anatomi RSUD Ulin Banjarmasin pada Juli 2022. Penelitian quasi eksperiment dengan rancangan Postest Only Control Group Design. Melalui pemeriksaan ketajaman warna reaksi dengan waktu inkubasi antibodi primer 8 menit, 12 menit dan 16 menit. Hasil pemeriksaan dianalisis statistik dengan uji Friedman untuk melihat ada atau tidaknya perbedaan pengaruh antar perlakuan, sedangkan untuk mengetahui waktu mana yang memberikan pengaruh dilakukan uji Wilcoxon dengan tingkat kepercayaan 95%. Hasil uji menunjukkan nilai signifikansi 0,000 (< 0,05) artinya adanya perbedaan bermakna antara waktu inkubasi antibodi primer Helicobacter pylori terhadap ketajaman warna reaksi. Waktu inkubasi yang berpengaruh terhadap ketajaman warna reaksi adalah 16 menit. Rerata nilai skor ketajaman warna reaksi tertinggi adalah 2,89 untuk waktu inkubasi 16 menit. Sehingga disarankan untuk menggunakan waktu inkubasi antibodi primer Helicobter pylori selama 16 menit pada pengecatan Autostaining Imunohistokimia. Kata Kunci : Waktu inkubasi, antibodi primer, ketajaman warna x ABSTRACT Effect of Helicobacter pylori Primary Antibody Incubation Time Against The Sharpness of Color Reaction in Autostaining Immunohistochemistry Examination of Gastric Biopsy in Gastritis Patients Researcher : Nani Jaini Advisors : Neni Oktiyani, Ahmad Muhlisin Primary antibody incubation is a protocol that must be done in the incubation Immunohistochemistry process which has problems causing poor staining intensity. Immunohistochemical staining protocol on gastric biopsies of gastritis patients using the Autostaining Roche Tissue Diagnostics Ventana BenchMark XT does not include the incubation time of the primary antibody Helicobacter pylori. Preliminary test with an incubation time of 16 minutes obtained the ideal reaction color and an incubation time of 20 minutes for the color of the overstaining reaction, while the incubation time was below 16 minutes and the reaction color was unknown. The purpose of this study was to determine the color acuity of the autostaining immunohistochemical examination of gastric biopsies in Helicobacter pylori gastritis patients with primary antibody incubation times of 8 minutes, 12 minutes and 16 minutes. The research was conducted at the Anatomical Pathology Laboratory Installation of Ulin Hospital, Banjarmasin in July 2022. This was a quasi-experimental study using the Posttest Only Control Group Design. By examining the color acuity of the reaction with primary antibody incubation times of 8 minutes, 12 minutes and 16 minutes. The results of the examination were analyzed statistically with the Friedman test to see whether or not there was a difference in effect between treatments, while to find out which time had an effect, the Wilcoxon test was carried out with a 95% confidence level. The test results showed a significance value of 0.000 (<0.05) meaning that there was a significant difference between the incubation time of the Helicobacter pylori primary antibody and the color acuity of the reaction. The incubation time that affects the color sharpness of the reaction is 16 minutes. The highest average reaction color sharpness score was 2.89 for 16 minutes of incubation time. So it is advisable to use Helicobter pylori primary antibody incubation time of 16 minutes in Autostaining Immunohistochemistry. Keywords : Incubation time, primary antibody, color acuity