Rifqoh, M.Sc Dosen Pembimbing 1 Tini Elyn Herlina, MM Ketua Penguji Jujuk Anton Cahyono, M.Sc Dosen Pembimbing 2 Siti Hamdiyyah Pengarang

ABSTRAK Gambaran Kualitas Reagen Malachite Green Dan Methylene Blue Pada Pemeriksaan Telur Cacing Dalam Feses Metode Kato-Katz Peneliti : Siti Hamdiyyah Pembimbing : Rifqoh, Jujuk Anton Cahyono Kecacingan merupakan salah satu penyakit di Indonesia yang sampai saat ini masih sangat tinggi. Metode pemeriksaan kecacingan salah satunya adalah metode kato-katz, reagen warna yang digunakan dalam pemeriksaan mikroskopis metode Kato-Katz yaitu malacite green dan methylene blue yang dilarutkan dengan aquades dan glycerol. Berfungsi sebagai larutan kato atau reagen, dengan perendaman selama 24 jam. Tujuan penelitian ini adalah mengetahui kualitas penyerapan warna selotif dan mengetahui kualitas warna latar belakang mikroskopis dengan reagen malachite green dan methylene blue. Jenis penelitian ini menggunakan Quasy experiment, dengan rancangan One group design, Penelitian ini menggunakan variasi waktu perendaman 1, 6, 12, 18 dan 24 jam. Sampel feses positif didapatkan dari Laboratorium Kesehatan Provinsi Kalimantan Selatan. Berdasarkan hasil pemeriksaan diketahui kualitas penyerapan warna malachite green pada selotif dengan variasi waktu 1, 6, 12, 18 dan 24 jam didapatkan warna light sea green, sedangkan kualitas penyerapan warna methylene blue pada selotif dengan variasi waktu 1, 6, 12, 18 dan 24 jam didapatkan warna cornflower blue. Hasil kualitas warna latar belakang pemeriksaan di mikroskop dengan reagen malachite green pada variasi waktu 1, 6, 12, 18 dan 24 jam didapatkan warna light sea green, sedangkan reagen methylene blue pada variasi waktu 1, 6, 12, 18 dan 24 jam didapatkan warna light cyan. Kesimpulan penelitian ini kualitas penyerapan warna dan kualitas warna latar belakang adalah reagen malachite green yang menghasilkan warna lebih baik dibandingkan reagen methylene blue. Bagi peneliti selanjutnya disarankan agar menambah variabel penelitian seperti kualitas pewarnaan reagen malachite green dan methylene blue pada warna telur cacing di mikroskop. Kata Kunci : Pemeriksaan Mikroskopis, Kato- Katz, Malachite green, Methylene blue ix ABSTRACT Description of the Quality Malachite Green and Methylene Blue Reagents in the Examination of Worm Eggs in Feces Kato-Katz Method Author : Siti Hamdiyyah Advisor : Rifqoh, Jujuk Anton Cahyono Worms is one of the diseases in Indonesia which is still very high. One of the worms examination methods is the kato-katz method, the color reagents used in the microscopic examination of the Kato-Katz method are malacite green and methylene blue dissolved in aquades and glycerol. Serves as a cathode solution or reagent, with immersion for 24 hours. The purpose of this study was to determine the quality of the color absorption of the adhesive tape and to determine the quality of the microscopic background color with malachite green and methylene blue reagents. This type of research uses a quasy experiment, with a One group design. This study uses a variation of immersion time of 1, 6, 12, 18 and 24 hours. Positive stool samples were obtained from the Health Laboratory of South Kalimantan Province. Based on the results of the examination, it is known that the quality of absorption of malachite green color on tape with time variations of 1, 6, 12, 18 and 24 hours obtained light sea green color, while the quality of absorption of methylene blue color on tape with time variations of 1, 6, 12, 18 and 24 the clock got cornflower blue color. The results of the quality of the background color examination in a microscope with malachite green reagent at time variations of 1, 6, 12, 18 and 24 hours obtained light sea green color, while methylene blue reagent at time variations of 1, 6, 12, 18 and 24 hours obtained color light cyan. The conclusion of this study is that the quality of color absorption and the quality of the background color is malachite green reagent which produces better color than methylene blue reagent. For further researchers, it is recommended to add research variables such as the quality of the staining of malachite green and methylene blue reagents on the color of worm eggs in the microscope. Keywords : Microscopic Examination, Kato-Katz, Malachite green, Methylene blue