Dra. Anny Thuraidah, Apt.,MS. Dosen Pembimbing 1 H. Haitami, M.Sc Ketua Penguji Dra. Ratih Dewi Dwiyanti, M. Kes Dosen Pembimbing 2 Muhammad Ridha Pengarang

ABSTRAK Efektivitas Ekstrak Rimpang Lengkuas Merah (Alpinia purpurata (Vieill.) K. Schum) sebagai Hand Sanitizer terhadap Penurunan Angka Kuman Peneliti: Muhammad Ridha Pembimbing: Anny Thuraidah, Ratih Dewi Dwiyanti Tangan adalah bagian dari tubuh manusia yang biasanya menjadi media penularan berbagai mikroorganisme patogen seperti bakteri, virus, dan jamur. Transmisi penyakit melalui tangan dapat diminimalisasi dengan menjaga kebersihan tangan, misalnya selalu menggunakan hand sanitizer. Lengkuas merah (Alpinia purpurata (Vieill.) K. Schum) mengandung sifat antibakteri sehingga dapat mengurangi pemakaian alkohol pada hand sanitizer. Tujuan penelitian ini adalah untuk mengetahui efektivitas ekstrak rimpang lengkuas merah sebagai hand sanitizer terhadap penurunan angka kuman. Jenis penelitian yang digunakan adalah true experiment dengan rancangan Pretest-Posttest Control Design, yang terdiri atas 7 perlakuan sebelum dan setelah menggunakan hand sanitizer ekstrak rimpang lengkuas merah formula 1, formula 2, formula 3, formula 4, dan formula 5 beserta kontrol positif (formula X) dan kontrol negatif (air mengalir) dengan 3 kali pengulangan. Hasil penelitian menunjukkan hand sanitizer ekstrak rimpang lengkuas merah yang mampu menurunkan angka kuman hanya dari formula 2 dan formula 3. Formula 2 menurunkan angka kuman sebesar 3,26×102 CFU/mL menjadi 1,5×101CFU/mL, sedangkan setelah pemberian formula 3 angka kuman sebesar 2,18×105CFU/mL turun menjadi 7,87×103CFU/mL. Dari hasil penelitian ini disimpulkan hand sanitizer ekstrak rimpang lengkuas merah efektif menurunkan angka kuman, dengan efektivitas tertinggi sebesar 96,4% pada formula 3. Untuk penelitian selanjutnya diharapkan untuk melakukan pengujian secara in vitro terlebih dahulu pada bakteri mikroflora normal dan melihat apakah ada aktivitas antibakteri pada hand sanitizer ekstrak rimpang lengkuas merah. Kata kunci: ekstrak, rimpang lengkuas merah, hand sanitizer, angka kuman ABSTRACT Effectiveness of Red Galangal Rhizome Extract (Alpinia purpurata (Vieill.) K. Schum.) as a Hand Sanitizer on Reducing Germ Numbers Researcher: Muhammad Ridha Advisors: Anny Thuraidah, Ratih Dewi Dwiyanti Hands are part of the human body which are usually a medium for transmission of various pathogenic microorganisms such as bacteria, viruses, and fungi. Transmission of disease through hands can be minimized by maintaining hand hygiene, for example always using hand sanitizer. Red galangal (Alpinia purpurata (Vieill.) K. Schum) contains antibacterial properties so that it can reduces the use of alcohol in hand sanitizers. The purpose of this study was to determine the effectiveness of red galangal rhizome extract as a hand sanitizer to reduce germ numbers. The type of research used is a true experiment with a Pretest-Posttest Control Design, which consists of 7 treatments before and after using hand sanitizers with red galangal rhizome extract of formula 1, formula 2, formula 3, formula 4, and formula 5 along with positive control (formula X) and negative control (water) with 3 repetitions. The results showed that the red galangal rhizome extract hand sanitizer was able to reduce the number of germs only from formula 2 and formula 3. Formula 2 reduced the germ number by 3.26×102 CFU/mL to 1.5×101CFU/mL, while after giving formula 3 the germ numbers was 2.18×105 CFU/mL decreased to 7.87×103 CFU/mL. From the results of this study, it was concluded that the hand sanitizer of red galangal rhizome extract was effective in reducing the number of germs, with the highest effectiveness of 96.4% in formula 3. For further research, it is expected to conduct in vitro tests first on normal microflora bacteria and see if there are antibacterial activities in hand sanitizer red galangal rhizome extract. Keywords: extract, red galangal rhizome, hand sanitizer, germ numbers