Tini Elyn Herlina, MM Dosen Pembimbing 2 Wahdah Norsiah, M.Si Dosen Pembimbing 1 Dinna Rakhmina, S.Si., M.Sc Ketua Penguji Sahri Rahman Pengarang

ABSTRAK Perbandingan Hasil Metode ICT dan ELISA untuk Deteksi Anti-HBs pada Tenaga Kesehatan Peneliti : Sahri Rahman Pembimbing : Wahdah Norsiah, Tini Elyn Herlina Berdasarkan Peraturan Menteri Kesehatan Republik Indonesia Nomor 53 Tahun 2015 petugas kesehatan merupakan kelompok berisiko tinggi terinfeksi Hepatitis B dan menjadi masalah yang perlu mendapat perhatian, dan vaksinasi cara paling efektif untuk mengurangi faktor risiko tersebut sehingga untuk mengetahui kadar antibodi antiHBs pada tenaga kesehatan perlu dilakukan pemeriksaan anti-HBs. Tujuan penelitian ini untuk mengetahui perbandingan hasil antara metode ICT dengan ELISA dalam pemeriksaan anti-HBs pada tenaga kesehatan. Penelitian ini bersifat deskriptif dengan rancangan cross-sectional. Populasi pada penelitian ini adalah tenaga kesehatan yang sudah divaksin Hepatitis B di Puskesmas Wilayah Kabupaten Banjar dan 25 tenaga kesehatan digunakan sebagai sampel penelitian. Sampel diambil dengan teknik purposive sampling. Antibodi anti-HBs dalam serum diukur menggunakan Immunochromatography Test (ICT) dan Enzyme-Linked Immunosorbent Asay (ELISA). Hasil penelitian menunjukkan pada metode ICT terdapat 19 orang (76%) responden dinyatakan reaktif dan 6 orang (24%) responden dinyatakan non reaktif, sedangkan metode ELISA terdapat 15 orang (60%) responden dinyatakan respon imun kuat ( > 100 mIU/mL ) dan 10 orang (40%) responden dinyatakan respon imun lemah ( 10 – 100 mIU/mL ). Data dianalisis dengan uji statistik Chi Square dengan nilai p-value yang didapat sebesar 0,225 sehingga lebih besar dari taraf kemaknaan 0,05, maka dapat disimpulkan bahwa tidak terdapat perbedaan hasil yang signifikan antara metode ICT dan ELISA . Saran kepada petugas kesehatan yang memiliki titer antibodi yang rendah (10-100 mIU/mL) harus divaksinasi ulang untuk meningkatkan titer anti-HBs, dan bagi peneliti selanjutnya lebih memperbanyak jumlah responden dengan subjek yang berbeda. Kata kunci : Anti-HBs, Tenaga Kesehatan, Immunochromatography Test, EnzymeLinked Immunosorbent Asay ix ABSTRACT Comparison The Results of ICT and ELISA Method for anti-HBs Detection in Health Workers Researcher: Sahri Rahman Supervisor: Wahdah Norsiah, Tini Elyn Herlina According to Regulation No. 53 of 2015 of the Minister of Health of the Republic of Indonesian, a high risk of HBV infection in health workers is a problem that requires attention, and vaccination is effective way to reducing these risk factors so that it is necessary to determine the level of anti-HBs antibodies in health workers anti-HBs test. The purpose of this study was to compare the results between the ICT method and ELISA in the examination of anti-HBs in health workers. This research is descriptive with a cross-sectional design. The population in this study were health workers who had been vaccinated against Hepatitis B at the Banjar District Health Center and 25 health workers were used as research samples. Samples were taken by the purposive sampling technique. Anti-HBs antibody in serum were measured using Immunochromatography Test (ICT) and Enzyme-Linked Immunosorbent Assay (ELISA). The results of the study showed that in the ICT method there were 19 (76%) respondents declared reactive and 6 (24%) respondents declared non-reactive, while the ELISA method contained 15 (60%) respondents who stated strong immune response (> 100 mIU /mL) and 10 people (40%) of the respondents stated that the immune response was weak (10-100 mIU/mL). Data analysis with Chi Square statistical test with p-value obtained is 0.225 so it is greater than the significance level of 0.05, conclusion that there is no significant difference in results between the ICT method and ELISA. Suggestions to health workers who have low antibody titers (10-100 mIU/mL) should be revaccinated to increase anti-HBs titers, and for further researchers to more respondents with different subjects. Keywords: Anti-HBs, Health Workers, Immunochromatography Test, Enzyme-Linked Immunosorbent Asay