Neni Oktiyani, M.Sc Ketua Penguji H. Ahmad Muhlisin, M.Kes Dosen Pembimbing 1 Wahdah Norsiah, M.Si Dosen Pembimbing 2 Nili Norhafiizhah Pengarang

ABSTRAK Perbedaan Hasil Morfologi Sel Eritrosit dan Sel Leukosit Metode Romanowsky Menggunakan Buffer Fosfat pH 6,8 dan Air Mineral Beremerek Nasional Penulis : Nili Norhafiizhah Pembimbing : Ahmad Muhlisin, Wahdah Norsiah Sediaan darah tipis merupakan pemeriksaan darah rutin menggunakan teknik mikroskopis untuk mengamati morfologi sel darah bahkan komponen lain didalam darah yang memberikan informasi tentang keadaan hematologik seseorang. Pewarnaan sediaan darah tepi diperlukan untuk memudahkan dalam menentukan berbagai jenis sel dan juga dalam mengevaluasi morfologi sel. International Council for Standardization in Haematology (ICSH) merekomendasikan pewarnaan Romanowsky sebagai metode pewarnaan yang digunakan dalam pembuatan sedian darah tepi karena pewarnaan ini memberikan hasil memuaskan pada sedian darah tepi. Larutan pH pengencer pewarnaan sangat berpengaruh terhadap kualitas sediaan darah tepi, dimana batas pH buffer fosfat yang biasanya digunakan sebagai pengencer pewarnaan yaitu pH 6,8-7,2 Menurut Standar Nasional Indonesia (SNI) 01-3553 tahun (2006) air mineral mempunyai batasan pH optimum berkisar 6,0-8,5, sehingga air mineral dapat digunakan sebagai alternatif pengganti larutan buffer. Tujuan penelitian mengetahui perbedaan hasil morfologi sel eritrosit dan sel leukosit Metode Romanowsky menggunakan buffer fosfat pH 6,8 dan air mineral bermerek Nasional. Penelitian ini merupakan penelitian observasional laboratorium dengan rancangan comparrative study. Hasil penelitian morfologi sel eritrosit dan sel leukosit darah kontrol Normal, Low, dan High mengunakan buffer fosfat pH 6,8 (A), air mineral bermerek Nasional C, D, F, dan G hasil pewarnaan baik dan air mineral bermerek Nasional B dan E hasil pewarnaan tidak baik. Berdasarkan uji Wilxocon morfologi sel eritrosit dan sel leukosit didapatkan sig >0,05 untuk air mineral bermerek Nasional C, D, F, dan G terhadap buffer fosfat pH 6,8 menunjukan tidak ada perbedaan bermakna. Disimpukan bahwa air mineral bermerek Nasional dapat digunakan sebegai pengencer pewarnaan sediaan darah tipis. Kata kunci: Morfologi Eritrosit, Morfologi Leukosit, Romanowsky, Air Mineral Bermerek Nasional ABSTRACT Differences in Morphological Results of Erythrocytes and Leukocytes Using Romanowsky’s Method Using Phosphate Buffer pH 6,8 and National Branded Mineral Water Author : Nili Norhafiizhah Advisor : Ahmad Muhlisin, Wahdah Norsiah Thin blood smear is a routine blood examination using microscopic techniques to observe the morphology of blood cells and even other components in the blood that provide information about a person's hematological condition. Staining of peripheral blood preparations is needed to make it easier to determine various types of cells and also to evaluate cell morphology. The International Council for Standardization in Hematology (ICSH) recommends Romanowsky staining as the staining method used in the manufacture of peripheral blood smears because this stain gives satisfactory results in peripheral blood smears. The pH of the staining diluent is very influential on the quality of peripheral blood preparations, where the pH limit of phosphate buffer which is usually used as a staining diluent is pH 6.8-7.2 According to the Indonesian National Standard (SNI) 01-3553 years (2006) mineral water has a limit The optimum pH ranges from 6.0 to 8.5, so mineral water can be used as an alternative to buffer solutions. The purpose of this study was to determine the differences in the morphology of erythrocytes and leukocytes. The Romanowsky method used phosphate buffer pH 6.8 and National branded mineral water. This research is a laboratory observational study with a comparative study design. The results of the study of morphology of erythrocyte cells and leukocyte cells in Normal, Low, and High control blood used phosphate buffer pH 6.8 (A), mineral water with National brands C, D, F, and G with good staining results and mineral water with National brands B and E staining results are not good. Based on the Wilxocon test, the morphology of erythrocyte and leukocyte cells was obtained sig > 0.05 for mineral water with National brands C, D, F, and G against phosphate buffer pH 6.8, which showed no significant difference. It was concluded that national branded mineral water could be used as a diluent for staining thin blood preparations. Keywords : Eryhrocyte Morphology, Leukocyte Morphology, Romanowsky, National Brand Mineral Water