Dra. Nurlailah, Apt., M.Si. Dosen Pembimbing 2 Rifqoh, M.Sc Dosen Pembimbing 1 Dra. Ratih Dewi Dwiyanti, M. Kes Ketua Penguji Rahmayunita Pengarang

ABSTRAK Efektivitas Ekstrak Etanol Daun Jeruk Sambal (Cytrus amblycarpa (Hassk) Ochse) Sebagai Biolarvasida terhadap Mortalitas Larva Nyamuk Aedes aegypti Peneliti : Rahmayunita Pembimbing : Rifqoh, Nurlailah Pengendalian vektor Aedes aegypti dalam pemberantasan larva berupa larvasida berbahan kimia dapat menimbulkan efek negatif seperti resistensi serta berbahaya bagi lingkungan dan manusia jika terus menerus digunakan sehingga diperlukan alternatif biolarvasida salah satunya daun jeruk sambal. Tujuan penelitian untuk mengetahui efektivitas ekstrak etanol daun jeruk sambal sebagai biolarvasida terhadap mortalitas larva nyamuk Aedes aegypti. Jenis penelitian adalah eksperimen dengan rancangan Posttest With Control Group Design. Subjek penelitian yang digunakan adalah larva nyamuk Aedes aegypti sebanyak 700 ekor. Hasil penelitian menunjukan kematian larva nyamuk Aedes aegypti setelah terpajan 24 jam ekstrak daun jeruk sambal konsentrasi 0,5%, 1%, 1,5%, 2%, dan 2,5% secara berturut-turut adalah 48%, 58%, 71%, 87%, dan 100%. Setelah terpajan 48 jam jumlah kematian larva secara keseluruhan adalah 100%. Berdasarkan hasil uji regresi yang dilakukan didapatkan efektifitas ekstrak daun jeruk sambal sebesar 83,8%, dan nilai LC90 didapatkan pada konsentrasi 0,181802%. Sehingga disimpulkan bahwa ekstrak etanol daun jeruk sambal (Cytrus amblycarpa) efektif terhadap kematian larva nyamuk Aedes aegypti. Bagi peneliti selanjutnya, disarankan melakukan penelitian dengan pelarut yang berbeda seperti pelarut non polar dengan menggunakan metode ekstraksi yang lain. Kata Kunci: Daun Jeruk Sambal (Cytrus amblycarpa), Aedes aegypti ABSTRACT Effectiveness of the Ethanol Extract of Sambal Jeruk Leaf (Cytrus amblycarpa (Hassk) Ochse) as a Biolarvicide against Mortality of Aedes aegypti Mosquito Larvae Author : Rahmayunita Advisor : Rifqoh, Nurlailah Control of Aedes aegypti vectors in larval eradication in the form of chemical larvicides can cause negative effects such as resistance and is harmful to the environment and humans if used continuously, so alternative biolarvicides are needed, one of which is sambal lime leaves. The purpose of the study was to determine the effectiveness of the ethanolic extract of chili lime leaves as a biolarvicide on the mortality of Aedes aegypti mosquito larvae. This type of research is an experiment with a Posttest With Control Group Design. The research subjects used were 700 Aedes aegypti mosquito larvae. The results showed that the mortality of Aedes aegypti mosquito larvae after 24 hours of exposure to sambal lime leaf extract concentrations of 0.5%, 1%, 1.5%, 2%, and 2.5%, respectively, was 48%, 58%, 71 %, 87%, and 100%. After 48 hours of exposure, the overall larval mortality was 100%. Based on the results of the tests carried out, the effectiveness of sambal lime leaf extract was 83.8%, and the LC90 value was obtained at a concentration of 0.181802%. These results indicate that the ethanol extract of sambal lime leaves (Cytrus amblycarpa) is effective against the death of Aedes aegypti mosquito larvae. For further researchers, it is recommended to conduct research with different solvents such as non-polar solvents using other extraction methods. Keywords : Sambal Lime Leaf (Cytrus amblycarpa), Aedes aegypti